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R&D Systems panel a r d systems cat
Panel A R D Systems Cat, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 759 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/panel+a+r+d+systems+cat/Proteome+Profiler+Mouse+Cytokine+Array+Kit%2C+Panel+A/pm41027427-218-97-99
Average 96 stars, based on 759 article reviews

panel a r d systems cat - by Bioz Stars, 2026-11

96/100 stars

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Enzyme-linked Immunosorbent Assay:

Article Title: Caspase-8 inhibition improves the outcome of bacterial infections in mice by promoting neutrophil activation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc BlockTM) Clone 2.4G2 (RUO) BD Biosciences Cat #553142 PE Rat Anti-Mouse Ly-6G Clone 1A8 BD Biosciences Cat #551461 PE Rat IgG2a, k Isotype Control BD Biosciences Cat #553930 F4/80 Monoclonal Antibody (BM8), eFluorTM 450, eBioscienceTM Invitrogen Cat #48-4801-82 Rat IgG2a kappa Isotype Control (eBR2a), eFluorTM 450, eBioscienceTM Invitrogen Cat #48-4321-82 IL-1 beta (Pro-form) Monoclonal Antibody (NJTEN3), APC, eBioscienceTM Invitrogen Cat #17-7114-80 Rat IgG1 kappa Isotype Control (eBRG1), APC, eBioscienceTM Invitrogen Cat #17-4301-82 PE Rat Anti-Mouse CD19 Clone 1D3 BD Biosciences Cat #553786 CD3 Monoclonal Antibody (17A2), Alexa FluorTM 700, eBioscienceTM Invitrogen Cat #56-0032-82 Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa FluorTM 700, eBioscienceTM Invitrogen Cat #56-4031-80 CD117 (c-Kit) Monoclonal Antibody (2B8), eFluor 450 Invitrogen Cat #48-1171-82 Rat IgG2b kappa Isotype Control (eB149/10H5), eFluorTM 450, eBioscienceTM Invitrogen Cat #48-4031-82 Ly-6G Monoclonal Antibody (1A8-Ly6g), Functional Grade, eBioscienceTM Invitrogen Cat #16-9668-82 Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscienceTM Invitrogen Cat #16-4321-82 Goat polyclonal to beta Actin- Loading Control antibody Abcam Cat #ab8229 Mouse IL-1 beta/IL-1F2 Antibody R&D Systems Cat #AF-401-NA Phospho-RIP (Ser166) (E7G6O) Rabbit mAb Cell Signaling technology Cat #53286 RIP (D94C12) XP Rabbit mAb Cell Signaling technology Cat #3493 Polyclonal Swine Anti-Rabbit Immunoglobulins/HRP Dako Code number #P0399 Polyclonal Rabbit anti-Goat IgG HRP-conjugated Antibody R&D Systems Cat #HAF017 Bacterial and virus strains Streptococcus agalactiaetyping strain H36B ATCC ATCC 8057 Streptococcus pneumoniaeserotype 2 (strain D39) University of Siena, Italy NCTC 7466 Klebsiella pneumoniae AC133 University of Messina, Italy N/A Chemicals, peptides, and recombinant proteins Z-IETD-FMK (Caspase-8 Inhibitor) Sellckchem (Selleck Chemicals LLC) Cat #S7314 Z-VAD-FMK (Pan-caspase Inhibitor) Sellckchem Cat #S7023 Z-YVAD-FMK (Caspase-1 Inhibitor) Sellckchem Cat #S8507 Dimethyl Sulfoxide (DMSO) Sigma-Aldrich SKU #D8418 Todd-Hewitt broth (THB) Oxoid Cat #CM0189 LB broth, Miller Sigma-Aldrich SKU #L3152 Trypticasein Soy Broth (TSB) EP/USP/ISO Condalab Cat #1224 (Continued on next page) Cell Reports Medicine 4, 101098, July 18, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse CXCL1/KC DuoSet ELISA R&D Systems Cat #DY453 Mouse TNF-alpha DuoSet ELISA R&D Systems Cat #DY410 Mouse IL-18 DuoSet ELISA R&D Systems Cat #DY7625 Mouse IL-1 alpha/IL-1F1 DuoSet ELISA R&D Systems Cat #DY400 Proteome Profiler Mouse Cytokine Array Kit, Panel A R&D Systems Cat #ARY006 Bio-Plex ProTM Human Chemokine Panel, 40-Plex Bio-Rad Cat #171AK99MR2 Pierce LDH Cytotoxicity Assay Kit ThermoFisher Scientific Cat #88954 Micro BCATM Protein Assay Kit ThermoFisher Scientific Cat #23235 Pyrochrome LAL Chromogenic Endotoxin Testing Reagent Associates of Cape Cod, Inc. Cat #C1500 Caspase-Glo 8 Assay System Promega Cat #G8200 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation Invitrogen Cat #L34957 eBioscienceTM Intracellular Fixation & Permeabilization Buffer Set Invitrogen Cat #88-8824-00 RNeasy Mini Kit QIAGEN Cat #/ID: 74004 M-MLV Reverse Transcriptase Invitrogen Cat # 28025013 Experimental models: Organisms/strains Mouse: C57BL/6 Charles River Laboratories Strain Code 027 Mouse: CD1 IGS Charles River Laboratories Strain Code 022 Mouse: Casp1/11 / Max Planck Institute, Berlin, Germany N/A Mouse: Ripk3 / Genentech, Inc., CA, USA N/A Mouse: Ripk1 D138N University of Cologne, Germany N/A Mouse: Mlkl / The Walter and Eliza Hall Institute of Medical Research, Australia N/A Mouse:Mlkl / Casp8 / University Health Network, Toronto, ON, Canada N/A Mouse: Ifnb1 / Lund University, Sweden N/A Mouse:Myd88 / Osaka University, Japan N/A Mouse: Ticam1 / (TRIF KO) Osaka University, Japan N/A Mouse: Nlrp3 / Genentech, Inc., CA, USA N/A Software and algorithms GrapdhPad Prism GraphPad Software, Inc. RRID:SCR_002798 (http://www.graphpad.com/) Image LabTM Software Bio-Rad Cat #1709690 i-controlTMSoftware for Infinite 200 PRO plate reader TECAN https://lifesciences.tecan.com/ CFX Maestro Software for CFX Real-Time PCR Instruments Bio-Rad Cat #12013758 FlowJo software TreeStar https://www.flowjo.com/ Other BD TrucountTM Absolute Counting Tubes BD Biosciences Cat #340334 Precision Plus ProteinTM WesternCTM Blotting Standard Bio-Rad Cat #1610376 Bolt 4 to 12%, Bis-Tris Mini Protein Gel Invitrogen Cat #NW04120B0X Immun-Blot PVDF Membrane Bio-Rad Cat #1620177 96 Well TC-Treated Microplates Sigma-Aldrich SKU CLS3599 (Continued on next page) Cell Reports Medicine 4, 101098, July 18, 2023 e3 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER 96-well microplates with U-bottom, cellGradeTM BRANDplates VWR International Cat #781960 Corning Costar TC-Treated Multiple Well Plates Sigma-Aldrich SKUCLS3516 Corning Costar TC-Treated Multiple Well Plates Sigma-Aldrich SKU CLS3512 Corning Costar TC-Treated Multiple Well Plates Sigma-Aldrich SKUCLS3524 GentleMACSTM M Tubes Miltenyi Biotec.

Article Title: Non-cell-autonomous tumor promotion in DICER1 cancer predisposition.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Discovery OmniMap anti-Rabbit HRP conjugate Roche Cat #760-4311; RRID: AB_2811043 Bond Polymer Refine Detection kit Leica Cat # DS9800; RRID: AB_2891238 Bacterial and virus strains Streptococcus pneumoniae: serotype 4 strain: TIGR4 Rosch Laboratory strain N/A Mouse-adapted A/California/04/2009 H1N1 Influenza Virus Schultz-Cherry Laboratory strain N/A Biological samples Sorted cells from ASD+/- and ASD+/+ tumor stroma for microarray analysis This paper N/A Mononuclear cells from ASD+/- and ASD+/+ tumor and tumor stroma for single cell RNA-seq This paper N/A Human DICER1+/+ and DICER1+/- FN-RMS Samples This paper; International Pleuropulmonary Blastoma/DICER1 Registry N/A Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium, DMEM high glucose HyClone Cat #SH30243.01 USDA Import Tested Fetal Bovine Serum (FBS) HyClone Cat #SH30910.03 Antibiotic/ antimycotic Sigma-Aldrich Cat #A5955 Roswell Park Memorial Institute (RPMI) 1640 Medium Gibco Cat #11835055 Neurobasal Media Invitrogen Cat #21103049 EGF R&D Systems Cat #236-EG b-FGF R&D Systems Cat #3718-FB B27 Gibco Cat #17504044 GlutaMAX Gibco Cat # 35050-061 QIAzol lysis reagent Qiagen Cat #79306 Collagenase B Roche Cat #11088831001 Dispase II Roche Cat #04942078001 Accumax Solution Sigma-Aldrich Cat #2026-02-28 Matrigel Matrix Corning Cat #354234 Live/Dead Blue Thermo Fisher Cat #L34962 Bovine serum albumin (BSA) Sigma-Aldrich Cat #A7159 Histoclear National Diagnostics Cat # HS-200 Fc Receptor Blocker Innovex Cat #NB309 FuGENE6 Promega Cat #E2691 LL-37 Tocris Cat #5213 Erlotinib MedChemExpress Cat #HY-50896 Linsitinib MedChemExpress Cat #HY-10191 GSK199 Cayman Chemical Cat #17489 cOmpleteTM Protease Inhibitor Cocktail Roche Cat #11697498001 PhosSTOP Roche Cat #4906845001 Triton X-100 Sigma-Aldrich Cat #T8787 ACK Lysing Buffer Gibco Cat #A1049201 Complement Component C5a Biotechne Cat #2150-C5-025/CF ProLong Diamond Antifade Mountant Invitrogen Cat #P36961 Ficoll-Paque Plus GE Healthcare 17-1440-02 Phorbol 12-myristate 13 acetate (PMA) Tocris Cat #1201 A23187 Cayman Chemical Cat #11016 (Continued on next page) ll OPEN ACCESSArticle Developmental Cell 61, 1–17.e1–e12, January 14, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER pHrodo E. coli BioParticles conjugate for phagocytosis Invitrogen Cat #P35361 Disulfiram (Tetraethylthiuram disulfide) Sigma Aldrich Cat #86720-50G Critical commercial assays miRNeasy Micro Kit Qiagen Cat #217084 miRNeasy mini Kit Qiagen Cat #217004 Superscript III First Strand cDNA Synthesis Kit Thermo Fisher Cat #18080051 Fast SYBR Green Master Mix Thermo Fisher Cat #4385610 TaqMan Fast Advanced Master Mix Thermo Fisher Cat #4444964 PicoPure DNA Extraction Kit Thermo Fisher Cat #KIT0103 DNeasy Blood and Tissue Kit Qiagen Cat #69504 Pierce BCA Protein Assay Kit Thermo Fisher Cat #23225 Proteome Profilers Mouse Cytokine Array Kit, Panel A R&D Systems Cat #ARY006 Mouse Complement C5a ELISA Kit Abcam Cat #ab193718 KAPA RNA HyperPrep Kit with RiboErase Roche Cat #08098131702 DCFDA/H2DFDA Cellular ROS Assay Kit Abcam Cat #ab113851 Neutrophil Myeloperoxidase Activity Assay Kit Cayman Chemical Cat #600620 Neutrophil Elastase Activity Assay Kit Cayman Chemical Cat #600610 Deposited data Microarray data – purified murine ASD+/- FN-RMS tumor stroma This paper GSE264568 Microarray data – purified murine ASD+/+ FN-RMS tumor stroma Drummond et al.12 GSE98059 scRNA-seq – purified murine ASD+/+ and ASD+/- FN- RMS tumor and stroma This paper GSE264567 Bulk RNA-seq – human DICER1+/+ and DICER1+/- FN-RMS tumors This paper GSE264566 Human FN-RMS bulk RNA-seq from the St. Jude Pediatric Cancer Genome Project and Genomes 4 Kids Project https://pecan.stjude.cloud/ N/A T cell exhaustion gene sets Wherry et al.58 GSE9650 Experimental models: Cell lines ..

LDH Cytotoxicity Assay:

Assay Development:

Staining:

Article Title: Re-education of Tumor-Associated Macrophages by CXCR2 Blockade Drives Senescence and Tumor Inhibition in Advanced Prostate Cancer
Article Snippet: 0609AFC25 Anti mouse-TNF-a (Recombinant) Peprotech Cat. 500-P64; Lot. .. 1111207 Critical Commercial Assays Senescence b-Galactosidase Staining Kit Cell Signaling Technology Cat. #9860S Proteome Profiler Mouse Cytokine Array Kit, Panel A R&D Systems Cat. ARY006 (Continued on next page) Cell Reports 28, 2156–2168.e1–e5, August 20, 2019 e1 Continued REAGENT or RESOURCE SOURCE IDENTIFIER EasySep PE Positive Selection Kit II Stem Cell Technology Catalog # 17684 Deposited Data Raw RNA-Seq Data This Paper GEO accession number: GSE125273 https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE125273 Experimental Models: Cell Lines LNCap/C4 ATCC ATCC CRL-3313 PC3 ATCC ATCC CRL-1435 22RV1 ATCC ATCC CRL-2505 TRAMP-C1 ATCC ATCC CRL-2730 THP-1 ATCC ATCC TIB-202 Experimental Models: Organisms/Strains Mouse; C57BL/6NCrl Charles River Strain Code: 027 Mouse; Ptenpc / (C57BL/6J background) Donation from the laboratory of Prof. Pier Paolo Pandolfi (Harvard) N/A Mouse; Ptenpc / ; Trp53pc / (C57BL/6J background) Donation from the laboratory of Prof. Pier Paolo Pandolfi (Harvard) N/A Oligonucleotides PTEN CRISPR/Cas9 KO Plasmid (m) Santa Cruz Biotechnology sc-422475 TNFR1 CRISPR/Cas9 KO Plasmid (m) Santa Cruz Biotechnology sc-423442-KO-2 RT-qPCR primers, see Table S1 ‘‘this paper’’ Sigma Aldrich Software and Algorithms Flow Jo_v10 Tree Star https://flowjo.com/ ImageJ NIH https://imagej.nih.gov/ij/ Cytoscape Cytoscape https://cytoscape.org/ Graph Pad Prism GraphPad Software https://www.graphpad.com LEAD CONTACT AND MATERIALS AVAILABILITY There are restrictions to the availability of the following reagent: goat polyclonal anti-pSTAT6 (sc-11762, Santa Cruz Biotechnology). ..

Reverse Transcription:

Software:

Maestro Software:

Real-time Polymerase Chain Reaction:

Membrane:

Selection:

RNA sequencing:

CRISPR:

Plasmid Preparation:

Quantitative RT-PCR:

cDNA Synthesis:

SYBR Green Assay:

DNA Extraction:

Bicinchoninic Acid Protein Assay:

ROS Assay:

Activity Assay:

Microarray:

Purification:

RNA Sequencing:

other:

Article Title: Glioblastoma induces the recruitment and differentiation of dendritic-like "hybrid" neutrophils from skull bone marrow.
Article Snippet: Article

Virus:

Article Title: Fibroblasts mobilize tumor cell glycogen to promote proliferation and metastasis
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Total glycogen synthase Cell Signaling Technology Cat#3886; RRID:AB_2116392 p-p38 (thr180/tyr182) Cell Signaling Technology Cat#9215; RRID:AB_331762 p-p38 (thr180/tyr182) Cell Signaling Technology Cat#4631; RRID:AB_331765 Total p38 Cell Signaling Technology Cat#9212; RRID:AB_330713 p-MAPKAPK-2 (thr334) Cell Signaling Technology Cat#3007; RRID:AB_490936 anti-rabbit IgG-HRP Cell Signaling Technology Cat#7074; RRID:AB_2099233 anti-mouse IgG-HRP Cell Signaling Technology Cat#7076; RRID:AB_330924 Actin Sigma-Aldrich Cat#A5441; RRID:AB_476744 V5 Life Technologies Cat#MA5–15253; RRID:AB_10977225 p-PGM1(Y353) GenScript Custom order Total PGM1 Santa Cruz Biotechnology Cat#sc-373796; RRID:AB_10918014 Total PGM1 Proteintech Cat#15161–1-AP; RRID:AB_2161415 PYGL Proteintech Cat#15851–1-AP; RRID:AB_2175014 Bacterial and Virus Strains NEB® 5-alpha Competent E. coli (High Efficiency) New England BioLabs Cat#C2987H Biological Samples Human carcinoma-associated fibroblasts This paper Human normal fibroblasts This paper Chemicals, Peptides, and Recombinant Proteins CP-91149 Sigma-Aldrich Cat#PZ0104 PH-797804 (p38α inhibitor) Selleck Chemical Cat# S2726 C13N15 Arginine Cambridge Isotope Laboratories Cat#CNLM-539-PK C13N15 Lysine Cambridge Isotope Laboratories Cat#CNLM-291-H- Collagenase type 3 Worthington Biochemical Cooperation Cat# LS004208 Hyaluronidase Worthington Biochemical Cooperation Cat# LS002592 2-NBDG Caymen Chemical Cat#11046 U- 13 C-glucose Cambridge Isotope Laboratories Cat#CLM-1396-PK Glycogen Hydrolysis Enzyme Caymen Chemical Cat#700483 CellRox Deep Red Reagent Invitrogen Cat# C10422 Matrigel BD Biosciences Cat#356231 Gateway LR Clonase II Enzyme Invitrogen Cat#11791–020 phosphoglucomutase Sigma-Aldrich Cat#P3397 glycogen Sigma-Aldrich Cat#G0885 NADP+ Sigma-Aldrich Cat#N0505 IL-6 PeproTech Cat#200–06 IL-8 PeproTech Cat#200–08 CXCL10 PeproTech Cat#300–12 CCL5 PeproTech Cat#300–06 Stattic MedChem Express Cat#HY-13838 Trametinib MedChem Express Cat#HY-10999 NSC23766 Sigma-Aldrich Cat#SML0952 H-89 Tocris Bioscience Cat#2910 14–22 amide Tocris Bioscience Cat#2546 Resazurin Sigma-Aldrich Cat#R7017 Diaphorase Sigma-Aldrich Cat#D5540 Glucose-6-phosphate dehydrogenase Sigma-Aldrich Cat#G7877 Critical Commercial Assays Glycogen Assay Kit BioVision Cat#K646–100 Q5 Site-Directed Mutagenesis Kit New England Biolabs Cat#E0554S Phosphoglucomutase Colorimetric Assay Kit BioVision Cat#K774–100 ATP/ADP ratio kit Sigma-Aldrich Cat#MAK135–1KT The Human Cytokine Array, Panel A R&D Systems Cat#ARY005 EnzyChrom L-Lactate Assay Kit BioAssay Systems Cat#ECLC-100 Deposited Data Phosphoproteomics ProteomeXchange PXD003574 Experimental Models: Cell Lines SKOV3ip1 Gordon Mills, M.D. .. Anderson Cancer Center, Houston, TX RRID:CVCL_8878 ES2 American Type Tissue Collection ATCC Cat# CRL- 1978, RRID:CVCL_3509 MDA-MB-231 American Type Tissue Collection ATCC Cat# HTB-26, RRID:CVCL_0062 RKO American Type Tissue Collection ATCC Cat# CRL- 2577, RRID:CVCL_0504 HeyA8-cGFP-Luc ( Mitra et al., 2012 ) N/A SKOV3ip1-cGFP-Luc ( Mitra et al., 2012 ) N/A TYK-nu Dr. Gottfried Koneczny, UCLA RRID:CVCL_1776 OVKATE Dr. Gottfried Koneczny, UCLA RRID:CVCL_3110 Caco-2 American Type Tissue Collection ATCC Cat# HTB-37, RRID:CVCL_0025 SNU-119 Korean Cell Line Bank KCLB Cat# 00119, RRID:CVCL_5014 PC-3 American Type Tissue Collection ATCC Cat# CRL- 1435, RRID:CVCL_0035 HEK293T Lucy Godley, University of Chicago RRID:CVCL_0063 Tyknu-V5-PGM1-WT this paper Tyknu-V5-PGM1-Y353F this paper SKOV3ip1-V5-PGM1-WT this paper SKOV3ip1-V5-PGM1-Y353F this paper Experimental Models: Organisms/Strains Athymic nude mice (Hsd: Athymic Nude- Foxn1 nu ) Envigo (Harlan) Oligonucleotides PGM1 siGENOME SMARTpool siRNA Dharmacon, GE Healthcare Cat#M-009569–02- 0005 PGM1 mutagenesis forward primer GATTGCTTTGttcGAGACCCCAAC Integrated DNA Technologies custom PGM1 mutagenesis reverse primer TTTGTAGCACTAGCCACC Integrated DNA Technologies custom MAPK14 siGENOME SMARTpool siRNA Dharmacon, GE Healthcare Cat# M-003512–06- 0005 Recombinant DNA pLX304 Addgene Cat#25890 pDONR221-PGM1 DNASU Plasmid Repository clone HsCD00039472 pLX304-V5-PGM1 WT this paper pLX304-V5-PGM1-Y353F this paper pLKO.1 TRC control Addgene Cat#10879 pLKO-shp38 Addgene Cat#52920 Software and Algorithms Prism software GraphPad RRID: SCR_002798 Incucyte Zoom software Essen Bioscience Microsoft Visual Basic 6.0 Thermo Fisher Scientific R package - QVALUE Bioconductor RRID:SCR_001073 ImageJ https://imagej.nih.gov/ij/ RRID:SCR_003070 Living Image Software Caliper Life Sciences RRID:SCR_014247 Open in a separate window KEY RESOURCES TABLE.

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